FluoBolt™-FIAs

FIANOSTICS develops and produces high-sensitivity fluorescence immunoassays based on Metal Enhanced Fluorescence for clinical research, which can be used on any fluorescence micro-plate reader. We also offer custom development of immunoassays and/or transfer of existing assays to our MEF-platform.

Metal Enhanced Fluorescence (MEF) offers the possibility to dramatically increase the analytical sensitivity of systems based on fluorescence detection. MEF is based on the fact that excitation light interacts with the electrons of metal nano–structures thus generating very high electromagnetic fields (Localised Surface Plasmons, LSPs ) Therefore, such structures are also called ”plasmonic structures” and the combination of (e.g, polymeric) support and structure is known as “plasmonic substrate”. These LSPs lead to an increase in emission output of fluorescent molecules (e.g. fluorescently labelled antibodies) when bound to surfaces with suitable nano-metal structures that can enhance the signal more than 100 times.

The unique features of FluoBolt™-Technology enables us to develop direct fluorescence immunoassays with the following benefits: High Sensitivity, Single Step Assay, No Enzym Substrate, Stable Signal over Time